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Thursday, August 6, 2020 | History

2 edition of Structural requirements for adenovirus DNA replication found in the catalog.

Structural requirements for adenovirus DNA replication

Chin-Hwa Hu

Structural requirements for adenovirus DNA replication

by Chin-Hwa Hu

  • 344 Want to read
  • 5 Currently reading

Published .
Written in English

    Subjects:
  • Adenoviruses.

  • Edition Notes

    Statementby Chin-Hwa Hu.
    The Physical Object
    Pagination70 leaves, bound :
    Number of Pages70
    ID Numbers
    Open LibraryOL15529096M

    28 Parvovirus DNA Replication Susan F. Cotmorel and Peter Tattersall’l structural) proteins in MVM. Genome usage in all parvoviruses is re- markably efficient, so that some protein sequences are encoded in over- or adenovirus, the infecting AAV genome integrates into the hostFile Size: KB. 6/6/ 3 van der Vliet, P.C., and Levine, A.J. DNA binding proteins specific for cells infected by adenovirus. Natl. New Biol.

    DNA replication accessory proteins include 3 ' -5 ' exonuclease, DNA primase, RNase H, 5 ' -3 ' exonuclease, DNA helicases, and DNA ligases. They are reviewed in other chapters in this book. aThe AdDBP increases the strand-displacement activity of the adenovirus-encoded DNA polymerase. bICP8 can also enhance processivity. 3 R 0 I cn s s 2 E. 3 0). Author Summary The cellular DNA damage response (DDR) network interprets the presence of replicating viral DNA genomes as DNA damage and strives to repair it, leading to inhibition of virus replication. Many DNA viruses, including adenovirus, evolved mechanisms to inhibit the DDR, thus increasing the efficiency of virus replication. In this study we identify a novel .

    Suggested Citation:"Site-specific integration by adeno-associated virus."National Academy of Sciences. (NAS Colloquium) Genetic Engineering of Viruses and Viral gton, DC: The National Academies Press. doi: / Challberg, M. D., Desiderio, S. V., & Kelly, T. J. (). Adenovirus DNA replication in vitro: Characterization of a protein covalently linked to nascent DNA strands. Proceedings of the National Academy of Sciences of the United States of America, 77(9 II), Cited by:


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Structural requirements for adenovirus DNA replication by Chin-Hwa Hu Download PDF EPUB FB2

Structural requirements for adenovirus DNA replication and the relationship between the cis and trans pathways for complementary strand replication was investigated.

Plasmids containing single or dual adenovirus origins, with or without inverted repeats, were specially constructed to mimic the structures of the adenovirus genome. Linear. Kenny MK, Hurwitz J () Initiation Structural requirements for adenovirus DNA replication book adenovirus DNA replication II.

Structural requirements using synthetic oligonucleotide adenovirus templates. J Biol Cited by: The initiation of adenovirus DNA replication is atypical in that the β-hydroxyl group of a serine residue in the precursor to the terminal protein (pTP), an to kDa polypeptide, acts as a primer in DNA replication.

Viral DNA replication can proceed bidirectionally and by single-strand displacement from either end of the DNA : Walter Doerfler. Replication of the adenovirus genome is catalysed by adenovirus DNA polymerase in which the adenovirus preterminal protein acts as a protein primer.

DNA polymerase and preterminal protein form a heterodimer which, in the presence of the cellular transcription factors NFI/CTFI and NFIII/Oct-1, binds to the origin of DNA by: Structural Features of the Viral DNA Linear double-stranded adenovirus DNA has been reported to sediment under neutral and alkaline conditions with sedimentation coefficients of s andrespectively, which corresponds to a molecular weight of x lo6 daltons (Doerfler and Kleinschmidt, ).

Adenovirus DNA replication Article Literature Review (PDF Available) in Current topics in microbiology and immunology February. ADVERTISEMENTS: In this article we will discuss about the replication of adenoviruses. Adenovirus replicates inside the nucleus (Fig. By the DNA replication process, the adenovirus multiplication cycle is separated into two phases i.e., an early phase and a late phase.

In both phases a primary transcript is formed which is spliced to produce monocistronic [ ]. Replication of origin containing adenovirus DNA fragments that do not carry the terminal protein.

Nucleic Acids Res. Apr 11; 11 (7)– [PMC free article] Tamanoi F, Stillman BW. Initiation of adenovirus DNA replication in vitro requires a specific DNA sequence.

Proc Natl Acad Sci U S A. Nov; 80 (21)–Cited by: Crystal structure of adenovirus DNAbinding protein Table I. Data collection and phasing summary Native KAu(CN)2 Pb(N03)2 K2PtC14 X (A). The viral DNA polymerase then uses a strand displacement mechanism, as opposed to the conventional Okazaki fragments used in mammalian DNA replication, to replicate the genome.

The late phase of the adenovirus lifecycle is focused on producing sufficient quantities of structural protein to pack all the genetic material produced by DNA : incertae sedis.

Adenovirus DNA Replication Rob C. Hoeben1 and Taco G. Uil2 1Department of Molecular Cell Biology, Leiden University Medical Centre, ZC Leiden, The Netherlands 2Crucell, CN Leiden, The Netherlands Correspondence: @ Adenoviruses have attracted much attention as probesto study biological processes such as.

The first eukaryotic DNA replication system for which both initiation and elongation could be reconstituted in vitro was that of the adenovirus. Three viral proteins are required for adenovirus genome replication: (1) adenovirus DNA polymerase (Ad pol), (2) the precursor terminal protein (pTP), and (3) the DNA-binding protein (DBP).Author: Rajesh Kumar, Aakansha Tiwari, Garima Pandey, Raj Narayan Trivedi, Amir Showkat Khan, Mumtesh Kumar.

Adenovirus genomes are linear, non-segmented double-stranded (ds) DNA molecules that are typically Kbp long, containing protein-coding genes. The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 protein-coding genes.

While the precise number and identity of genes varies among. complex that precedes initiation of viral DNA replication. ADENOVIRUS ORIGINS OF DNA REPLICATION The cis-acting DNA sequences that define ori, the origin of DNA replication, are located at the ends of the genome within the ITRs.

Covalently bound to each 5 ‘end of the DNA is a terminal protein (TP). Jacobo-Molina, P. Clark, S.H. Hughes, and E. Arnold, Crystallization of Human Immunodeficiency Virus Type 1 Reverse Transcriptase with and without Nucleic Acid Substrates, Inhibitors, and an Antibody Fab isms of DNA Polymerases:F.

Eckstein and J.B. Thomson, Phosphate Analogs for Study of DNA Polymerases.N.C. Brown and G.E. Wright. VIROLOGY() Kinetics of Adenovirus DNA Replication. Rate of Adenovirus DNA Replication JOHN W.

BODNAR AND GEORGE D. PEARSON1 Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon Accepted October 2, The rate of adenovirus DNA replication is constant throughout by:   Although the mechanism of adenovirus DNA replication is unique among the eukaryotic viruses, it bears many similarities with the protein-primed DNA replication of several bacteriophages such as the Bacillus subtilis bacteriophages ϕ29 and GA-1, the Escherichia coli bacteriophage PRD1, and the Streptococcus pneumoniae phage CP Similar to AdV.

Machine derived contents note: 1 Virion and Structure 1 --Molecular Evolution of Adenoviruses --M. BENKE, B. HARRACH 3 --Novel Partner Proteins of Adenovirus Penton --J. CHROBOCZEK, E. GOUT, A.-L. FAVIER, R. GALINIER 37 --Structural Studies on Adenoviruses --C.

SAN MARTFN, R.M. BURNETT 57 Viral Replication 95 --The Multifunctional Role of. The M, = 72, adenovirus-coded DNA-binding pro- tein is required for viral DNA replication and is in- volved in virus transcription and transformation.

This protein, as isolated from infected cells, is a phospho- protein with extensive charge heterogeneity. Between.

The precursor terminal protein pTP is the primer for the initiation of adenovirus (Ad) DNA replication and forms a heterodimer with Ad DNA polymerase (pol).

Pol can couple dCTP to pTP directed by the fourth nucleotide of the viral genome template strand in the absence of other replication proteins, which suggests that pTP/pol binding Cited by:. The E2 region encodes proteins necessary for replication of the viral genome: DNA polymerase, preterminal protein, and the kDa single-stranded DNA-binding protein (reviewed by de Jong et al., ).

Even though adenovirus replicates in the nucleus, it requires its own enzymatic machinery because of its chromosomal structure.DNA replication and recruitment of MRN to viral replication centers. We identified regions of Nbs1 that are differentially required for concatemer formation and inhibition of Ad DNA replication.

These results demon-strate that targeting of the MRN complex explains the redundant functions of E4orf3 and E4orf6 in promoting Ad DNA replication.Chapter 3 The fi(I/Y)XGGfl motif of Adenovirus DNA 37 polymerase affects Template DNA binding and the Transition from Initiation to Elongation Chapter 4 Molecular Architecture of Adenovirus DNA 51 Polymerase and Location of the Protein-Primer Chapter 5 Termination of Adenovirus DNA Replication 63 implicates a Role for the Terminal Protein.